Meet our grantees
Wild Animal Initiative funds academic research on high-priority questions in wild animal welfare.
The goal of our grants program is to fund research that deepens scientific knowledge of the welfare of wild animals in order to better understand how to improve the welfare of as many wild animals as possible, regardless of what causes the threats to their well-being.
We showcase our grantees and their projects here and will be adding more in the coming weeks and months.
Investigating ways to assess and improve the welfare of leopards captured in response to conflict, in Nepal
Grantee: Jessica Bodgener
Institutions: University of Kent, Wildlife Vets International
Project summary
In Nepal, the successful recovery of tiger populations is displacing leopards from protected parks and forcing them into urban areas. This has caused an increase in conflict with people and farmed animals. Organizations responding to conflicts have limited resources and must attempt to balance animal welfare and conservation aims with public safety and sociopolitical demands. This has resulted in an ad hoc approach, with frequent interventions but little opportunity to evaluate their success. This project will focus on validating non-invasive post-release monitoring techniques, including photogrammetric assessment of body condition and measurement of hair cortisol from fecal samples, to monitor the welfare impact of translocation of leopards away from conflict areas.
Grantee: Jessica Bodgener
Institution: University of Kent and Wildlife Vets International, United Kingdom
Grant amount: $16,850
Grant type: Small grants
Focal species: Leopard (Panthera pardus)
Conservation status: Vulnerable
Disciplines: Wildlife rehabilitation, animal welfare science
Research location: Nepal, United Kingdom
Project summary
Leopards are the most widespread and adaptable of the world’s big cats, occupying a wide variety of habitats ranging from remote rainforests to busy urban centers. In Nepal, the successful recovery of tiger populations is displacing leopards from protected parks and forcing them into more populated areas. Low densities of natural prey in these areas, coupled with an abundance of easily hunted feral dogs around settlements, have resulted in leopards colonizing urban and suburban environments. This has caused an increase in conflict with people, including the predation of farmed animals and occasional human attacks. Organizations responding to conflict events have limited resources and must attempt to balance animal welfare and conservation aims with public safety and sociopolitical demands. This has resulted in an ad hoc approach, with frequent interventions, but little to no opportunity to reflect and evaluate the impact or success of these actions. This project will focus on validating entirely non-invasive post-release monitoring techniques, including photogrammetric assessment of body condition and measurement of hair cortisol from fecal samples, to monitor the impact of translocation of leopards away from conflict areas on their health and welfare.
Why we funded this project
We funded this project because it addresses two of our research priorities by seeking to validate non-invasive monitoring techniques, including hair cortisol as a measurement of chronic stress (which may be more informative of welfare than short-term measures of stress physiology). Although it focuses on leopards, the validation of these methods should be transferable to other species. The researcher, Jess Bodgener, also seems to have a long-term interest in wild animal welfare, and has genuine partnerships in place to build capacity and regard for wild animal welfare in Nepal.
WellSeal: Non-invasive quantification of welfare in lactating harbour seals
Grantee: Jess Harvey-Carroll
Institution: University of Gothenburg
Project summary
Limited availability of harbor seal birthing sites leads to intraspecific competition, forcing mothers to choose when and for how long to forage, and resulting in trade-offs between maternal and offspring welfare. These trade-offs may be exacerbated by fishing, as harbor seals swim large distances to obtain food. This project will use camera trap footage and 3D modeling to assess the influence of maternal welfare on time spent away from pups, and pup welfare during periods of maternal absence. Welfare will be assessed based on body condition, an index of the optimality of haul-out position, and a record of positive and negative interactions. The assessment will be aggregated at colony level to compare welfare between colonies with varying access to food.
Grantee: Jess Harvey-Carroll
Institution: University of Gothenburg, Sweden
Grant amount: $30,000
Grant type: Small grants
Focal species: Harbour seal (Phoca vitulina)
Conservation status: Least concern
Disciplines: Population ecology, animal behavior, animal welfare science
Research location: Sweden
Project summary
Harbor seals require locations protected from adverse weather and other disturbances during the birthing season, when vulnerable pups are left on land while mothers forage. The limited availability of such sites leads to high intraspecific competition, in which larger seals are likely to dominate more desirable locations due both to their physical strength and their lower need to forage. Mothers must choose when and for how long to forage, leading to trade-offs between maternal and offspring welfare which may lay the foundation for the welfare of younger generations. Currently, little information is available on time spent foraging during the lactation period. As harbor seals are facing unprecedented stress from overfishing, seals are swimming increasingly large distances to obtain food. It is not known how this change affects the trade-off between maternal foraging and offspring care, and the resulting welfare of the pups. This project will use camera trap footage and state-of-the-art 3D modeling to non-invasively assess the influence of maternal welfare on the time spent away from the pups. Pup welfare during periods of maternal absence will also be assessed. Welfare will be assessed in terms of the Five Domains framework, based on individual body condition (health), an index of the optimality of their haul-out position (environment), and a record of positive and negative interactions (behavior). Such welfare assessment will be aggregated at a colony level to compare welfare between colonies with more and less access to food.
Why we funded this project
We funded this project because it seeks to evaluate welfare impacts of population density — likely a common cause of welfare issues — in a relatively abundant species, using a robust approach that is only possible thanks to the species-specific knowledge of the PI and her collaborators. For example, we value their use of image analyses to identify haul-out site suitability and welfare impacts on a fine spatial scale that takes into account both environmental and social factors. We also valued the focus on juvenile welfare and the conflict of interest that may arise between parent and offspring. Improving our understanding of the fundamental relationships between population density, parental care, and juvenile welfare will allow the results of this project to extend to other contexts.
Improving the welfare of translocated individuals - European mink as a case study
Grantee: Maria Diez Leon
Institution: University of London
Project summary
This project will assess how levels of two behaviors thought to track positive and negative welfare states — play behaviors and abnormal repetitive behaviors — influence post-release welfare metrics in two on-going reintroduction programs for the European mink. Fecal glucocorticoid metabolite levels will be measured to validate these behavioral indicators pre- and post-release. By differentially raising mink in conditions known to improve welfare, the researchers will investigate whether captive environments that promote play and decrease abnormal repetitive behaviors improve pre-release and post-release welfare, whether individual welfare state pre-release correlates with post-release welfare outcomes, and how the welfare of captive-born individuals differs from that of wild-born individuals.
Grantee: Maria Diez Leon
Institution: University of London, United Kingdom
Grant amount: $28,965
Grant type: Small grants
Focal species: European mink (Mustela lutreola)
Conservation status: Critically endangered
Disciplines: Wildlife rehabilitation, animal welfare science
Research location: Spain
Project summary
Conservation breeding programs do not proactively consider or even assess welfare across all stages, nor track the welfare of released individuals. We therefore lack data on how individual welfare state pre-release might affect welfare post-release. This project aims to fill the gap by assessing how levels of two behaviors thought to track positive and negative welfare states — play behaviors and abnormal repetitive behaviors — influence post-release welfare metrics in two on-going reintroduction programs for the European mink, and compare the welfare of released minks to individuals in an established “benchmark” wild population. Fecal glucocorticoid metabolite levels will also be measured to validate these behavioral indicators pre- and post-release. By differentially raising mink in conditions known to improve welfare, the researchers will investigate whether captive environments that promote play and decrease abnormal repetitive behaviors improve pre-release and post-release welfare, whether individual welfare state pre-release correlates with post-release welfare outcomes, and how the welfare of captive-born individuals differs from that of wild-born individuals.
Why we funded this project
We are interested in the comparison of long-term welfare outcomes between individuals born and protected in captivity and individuals born in the wild, which can help us understand how welfare issues differ between captive and wild environments and how much early-life experiences influence long-term welfare. The project’s objective of identifying improvements for the early-life care of animals who are to be released into the wild also helps us assess “headstarting,” a practice in which juveniles of a species are reared in captivity before being released at a less dangerous life stage, as a near-term intervention for improving the lifetime welfare of animals who have vulnerable juvenile stages but are relatively long-lived as adults. Finally, the applicant has a background in conservation but has demonstrated a long-term interest in wild animal welfare, such as attending an April 2022 workshop on animal sentience (LSE/Rethink Priorities).
Find Maria’s other project, studying American minks and Eurasian otters, here.
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Developing a consensus profile of wild animal welfare: integrating non-invasive monitoring of the gut microbiome with stress physiology and behavior
Grantee: Sam Sonnega
Institution: University of Massachusetts Dartmouth
Project summary
This project will characterize the gut microbiome of white-footed mice and investigate its relationship with their stress physiology and behavior. Mice will be trapped and fecal samples collected to measure glucocorticoid concentrations and gut microbiome composition. Concurrently, open-field trials will be conducted to assess individual variation in cognitive bias. Perception of predation risk will also be experimentally manipulated by exposing mice to playbacks of predator noises. By correlating the gut microbiome with behavioral and endocrine metrics, a consensus profile of the mice’s welfare will be developed that reflects the complexity of their responses to environmental perturbations, and how those responses can scale up to population and ecosystem level changes via the demographic effects of stress in a “landscape of fear.”
Grantee: Sam Sonnega
Institution: University of Massachusetts Dartmouth, United States
Grant amount: $29,130
Grant type: Small grants
Focal species: White-footed mice (Peromyscus leucopus)
Conservation status: Least concern
Disciplines: Physiology, animal welfare science, animal behavior
Research location: United States
Publications
Sonnega, S. and Sheriff, M.J. (2024). Harnessing the gut microbiome: a potential biomarker for wild animal welfare. Frontiers in Veterinary Science, 11. https://doi.org/10.3389/fvets.2024.1474028
Project summary
This project will characterize the gut microbiome of wild white-footed mice (Peromyscus leucopus) and investigate its relationship with their stress physiology and behavior, including how it changes in response to ecological pressures. While a growing literature from lab-based studies has demonstrated the link between the gut microbiome and regulation of host physiology and behavior, the generality of these findings in ecological contexts remains largely untested. As part of this project, mice will be trapped during different seasons and fecal samples will be collected from which to measure both glucocorticoid concentrations and gut microbiome composition. Concurrently, open-field trials will be conducted to assess individual variation in cognitive bias towards optimism or pessimism (a well-established behavioral indicator of affective state). The mice’s perception of predation risk will also be experimentally manipulated by exposing free-living mice to playbacks of predator noises. By correlating the gut microbiome with both behavioral and endocrine metrics, a consensus profile of the mice’s welfare will be developed that reflects the complexity of their responses to both predictable and unpredictable environmental perturbations, and how those responses can scale up to population and ecosystem level changes via the demographic effects of stress in a so-called “landscape of fear.”
Why we funded this project
We are interested in the development and validation of the gut microbiome as a welfare indicator. Every additional indicator strengthens the interpretation of others, but understanding the gut microbiome may be especially important because it is part of the causal chain linking what is going on in the animal’s brain to fecal metabolites, which are often analyzed as a non-invasive and time-integrated record of physiological stress. This project also links and builds on other gut microbiome research we have funded (by Melissa Bateson, Pablo Capilla-Lasheras, and Davide Dominoni).
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Assessing the Welfare of Wild Olive Baboons (Papio anubis) at the Uaso Ngiro Baboon Project, Laikipia, Kenya
Grantee: Monica Wakefield
Institution: Northern Kentucky University and Iowa State University
Project summary
This project aims to assess the welfare of wild baboons using a holistic approach. The study takes advantage of almost 40 years of long-term ecological, demographic, health, and behavioral information on >170 individually known baboons, and will examine how various factors such as age, sex, and social rank correlate with individual welfare measures such as incidence of injury and disease, body mass, and pro-social or agonistic behaviors. The annual scale of the dataset will also enable the researchers to test how events such as droughts have affected welfare during specific periods, and how these effects may have varied according to individual animals’ demographic profiles and biographies.
Grantee: Monica Wakefield
Institutions: Northern Kentucky University and Iowa State University, United States
Grant amount: $29,800
Grant type: Small grants
Focal species: Wild olive baboon (Papio anubis)
Conservation status: Least concern
Disciplines: Animal welfare science, animal behavior, primatology
Research location: Kenya, United States
Project summary
This project aims to assess the welfare of wild baboons using a holistic approach. The study takes advantage of almost 40 years of long-term ecological, demographic, health, and behavioral information on >170 individually known baboons, and will examine how various factors such as age, sex, and social rank correlate with individual welfare measures such as incidence of injury and disease, body mass, and pro-social or agonistic behaviors. The annual scale of the dataset will also enable the researchers to test how events such as droughts have affected welfare during specific periods, and how these effects may have varied according to individual animals’ demographic profiles and biographies.
Why we funded this project
We are excited by this project’s analysis of a long-term longitudinal dataset because of the importance of understanding how wild animals’ welfare varies with demographic factors such as age and sex, as these groups often face different challenges and have different ecological and behavioral requirements, and negative welfare impacts that fall on young individuals may also have ripple effects throughout their lives. We also appreciate this project’s holistic approach. The long-term monitoring means it is possible to consider not only the usual downstream welfare indicators based on health and behavior, but also upstream factors that might influence them, such as social interactions and exposure to predators.
Assessing the anthropogenic impacts, long-term health, and welfare of elasmobranch species within San Francisco Bay, California
Grantee: Meghan Holst
Institution: University of California, Davis
Project summary
The San Francisco Bay Estuary is used by individuals belonging to several elasmobranch populations during critical periods of their life history. But it is also dredged, used as a major harbor for ships, and fished, with sharks and their prey targeted. This project will measure stress physiology and blood contaminants to evaluate whether the health and welfare of elasmobranchs is threatened within San Francisco Bay. It will also use stable isotope analysis to evaluate the dietary needs and sensitivities of San Francisco Bay’s elasmobranch species to determine whether individuals are threatened with hunger due to fishing, and to map relationships within the ecosystem.
Grantee: Meghan Holst
Institution: University of California, Davis, United States
Grant amount: $19,200
Grant type: Small grants
Focal species: Shark (Selachimorpha sp.)
Conservation status: Near threatened
Disciplines: Animal welfare science, population ecology, community ecology, marine biology, ichthyology
Research location: United States
Project summary
Individuals belonging to several elasmobranch populations use the San Francisco Bay Estuary during critical periods of their life history. For example, the broadnose sevengill shark (Notorynchus cepedianus) is an apex predator that visits seasonally to pup. Pups then appear to reside in the estuary for several years before they join the adult population that migrates from Alaska to Baja, California. The San Francisco Bay also serves as a major harbor for container and cruise ships. To allow these large vessels to enter the bay, dredging occurs continuously within San Francisco Bay in the primary channel where many of the adult elasmobranchs also reside, potentially increasing contaminant exposure. Additionally, both commercial and recreational fishing occurs on shark species and their preferred prey, posing direct physiological impacts on elasmobranchs and constraints to their prey availability. Little has been done to evaluate potential unrecognized consequences of these activities on the health and welfare of elasmobranch species and the San Francisco Bay ecosystem. To address this gap in knowledge, this project will measure stress physiology and blood contaminants to evaluate whether the health and welfare of elasmobranchs is threatened within San Francisco Bay, and evaluate dietary needs of elasmobranch species within San Francisco Bay to determine whether individuals are threatened with hunger/starvation due to fishing of their preferred prey. Stable isotope analysis of the sharks and potential prey will also be used to more precisely identify their dietary needs and sensitivities, and ultimately map ecological relationships within the ecosystem.
Why we funded this project
We funded this project primarily because it addresses our interest in understanding the welfare implications of ecological system dynamics. The project also focuses on lifestage-specific ecological differences within the focal species, focusing on juveniles, which are the most numerous and often neglected. Additionally, the lead researcher is a PhD student who has an interest in wild animal welfare and has already demonstrated an aptitude for coordinating scientific research projects. The ability to write a compelling proposal at such an early research stage is very promising for their future career prospects.
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Physiological and behavioral effects of nicarbazin on feral urban pigeons (Columba livia)
Grantee: Jessica X. Wright-Lichter
Institution: Tufts University
Project summary
The oral contraceptive Nicarbazin (NCZ) has been shown to be effective in urban pigeons. However, despite evidence that it may limit birds’ ability to cope with high heat, the side-effects of prolonged exposure to NCZ on pigeons have not been studied. In this project, feral pigeons will be treated with NCZ and exposed to increased ambient temperature to assess their responses. Changes in corticosterone levels will be used as a physiological proxy for welfare. The researchers will also look for behavioral correlations by measuring the onset and frequency of heat-mediating behaviors. The behavioral effects of NCZ treatment alone will also be assessed by quantifying impacts on affiliative and aggressive behaviors, social status, and access to feed at bait sites.
Grantee: Jessica X. Wright-Lichter
Institution: Tufts University, United States
Grant amount: $30,000
Grant type: Small grants
Focal species: Urban pigeon (Columba livia domestica)
Conservation status: Least concern
Disciplines: Physiology, ornithology, animal welfare science
Research location: United States
Project summary
Nicarbazin (NCZ) is an oral contraceptive that has been shown to be effective in feral urban pigeons (Columba livia). However, although it is EPA-approved and widely marketed, no published studies examine the impact of prolonged exposure to NCZ on the pigeons beyond the contraceptive effect. This is concerning because evidence from agricultural use of the compound (where it was originally developed as an antiparasitic drug) suggests that NCZ exposure may limit the ability of birds to cope with high heat.
To study the impact of NCZ treatment on pigeon welfare, especially as a function of heat exposure, feral pigeons will be treated with NCZ for three weeks before being exposed to increased ambient temperature for four hours to assess their physiological and behavioral responses compared to a control group. This project will use changes in levels of the hormone corticosterone as a physiological proxy for welfare in exposed pigeons. The researchers will also look for behavioral correlations by measuring the onset and frequency of heat-mediating behaviors (e.g., panting, wing-spreading). The behavioral (and plausibly welfare-relevant) effects of NCZ treatment alone will also be assessed by quantifying impacts on affiliative and aggressive behaviors, social status, and access to feed at bait sites.
Why we funded this project
We funded this project because we see wildlife fertility control — particularly applied to urban pigeons — as a highly promising near-term intervention. We are especially interested in indirect welfare effects of fertility control (e.g.,on juvenile welfare and survival), but we have also been concerned by the lack of research on direct welfare impacts. This study will focus on direct welfare impacts while also providing some of the first data on NCZ’s effects on pigeon social dynamics, which are likely to be very important determinants of overall welfare. These data could then be used to guide the application of NCZ contraceptives (e.g., their seasonal timing) to maximize pigeon well-being while successfully controlling urban pigeon populations.
It takes guts to grow in the city: the role of the gut microbiome in the welfare of juvenile urban birds
Grantee: Pablo Capilla-Lasheras
Institution: University of Glasgow
Project summary
Approximately 30 million blue tits hatch every year in UK cities, but around 63% die as juveniles. One reason for this may be that juvenile birds in urban habitats tend to have low-quality diets, causing stunted growth and increased early-life mortality. Research suggests that gut microbiome composition may contain indicators of an animal’s welfare, but this has received little attention in a wild animal context. This project will combine telomere attrition, begging behavior, and other health metrics with a study of juvenile birds’ gut microbiomes to investigate how to improve the welfare of juvenile blue tits in urban areas. It will also test the potential for gut microbiome enrichment to improve the life experience of juvenile birds in urban habitats.
Grantee: Pablo Capilla-Lasheras
Institution: University of Glasgow, United Kingdom
Grant amount: $59,052
Grant type: Challenge grants
Focal species: Blue tits (Cyanistes caeruleus)
Conservation status: Least concern
Disciplines: Physiology, population ecology, animal welfare science, ornithology, animal behavior
Research location: United Kingdom
Publications
Reid, R., et al. (2024). The impact of urbanization on health depends on the health metric, life stage and level of urbanization: a global meta-analysis on avian species. Proceedings of the Royal Society B, 291(2027). https://doi.org/10.1098/rspb.2024.0617
Project summary
Amongst the novel stressors that wildlife face in cities, shifts in diet are prevalent and have negative consequences for welfare, particularly in juveniles. Research clearly shows that juvenile birds in urban habitats have low-quality diets, causing stunted growth and increased early-life mortality. For example, in the UK alone, approximately 30 million blue tits hatch every year in cities, but around 63% of them die as juveniles. This project will study juvenile blue tits and investigate how to improve their welfare in urban areas using an integrative approach that will combine metrics of animal welfare with the study of gut microbiomes. Human and captive animal studies suggest that the gut microbiome composition may contain indicators of an animal’s welfare, but this has received little attention in a wild animal context. This study will combine a physiological welfare indicator (telomere attrition), a behavioral welfare indicator (begging behavior), and other health metrics with a study of juvenile birds’ gut microbiomes to link poor-quality urban diets, gut microbiome composition, and welfare. The project will also test a potential near-term dietary intervention (gut microbiome enrichment) to improve the life experience of juvenile birds growing in urban habitats.
Why we funded this project
Welfare is shaped by, and in turn influences, numerous aspects of an animal’s phenotype. Individual welfare indicators offer only limited insights, but combining indicators across multiple domains is thought to be extremely important for triangulating affective state, or “true” welfare. Because the gut microbiome represents a partially distinct domain, understanding how it relates to welfare could therefore strengthen all of our other indicators when they are used in combination. We were especially interested in this project due to the inclusion of an intervention experiment that could be implemented in the near future. Finally, this project will support an early-career researcher with an interest in wild animal welfare.
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Density-Dependent Welfare in Wild Bird Social Networks: Linking resource distributions with disease dynamics
Grantee: Joshua Firth
Institution: University of Oxford
Project summary
This project will investigate how various potential density-dependent drivers of welfare interact and influence net welfare in two species of tit. Population density is expected to be directly related to infectious disease transfer and increased competition, but also to covary with processes that are potentially beneficial to welfare. The study will use historical data to determine how the relationship between population density and individual welfare is shaped by infectious disease, body condition, and mortality risk. The investigators will then manipulate density experimentally to test model outputs and determine potential causal links.
Grantee: Josh Firth
Institution: University of Oxford, United Kingdom
Grant amount: $99,466
Grant type: Challenge grants
Focal species: Great tits (Parus major), blue tits (Cyanistes caeruleus)
Conservation status: Least concern
Disciplines: Animal behavior, community ecology, infectious disease, population ecology, ornithology, bio/eco-informatics, ecological modeling, animal welfare science
Research location: United Kingdom
Project summary
This project seeks to understand the interplay among various potential density-dependent drivers of positive and negative welfare impacts to determine net welfare related to aggregation (increased density) in wild birds (two species of tit). Infectious disease transfer and increased competition, both likely to reduce welfare, are expected to be directly related to population density. However, population density also covaries with processes that are potentially beneficial to welfare, such as congregation around areas that provide high nutrition, various social benefits, opportunities for increased cooperation, or access to social information. The study will use long-term datasets to empirically determine how the relationship between population density and individual welfare is shaped by infectious disease, including the density-dependent relationship with disease spread. Additionally, using historical data, the project will test how population density, mediated by social contact, environment, and disease, determines individuals’ body condition and mortality risk. Based on the results of this observational phase of the study, the investigators will then manipulate density experimentally, to test model outputs and determine potential causal links.
Why we funded this project
This project brings two unique advantages. First, the investigators have access to a population that has been subject to intensive monitoring for decades, meaning that much of the relevant ecological context is known and there is historical data to analyze retrospectively. Secondly, in part due to this long-term monitoring, the birds are tagged and observed at feeding stations equipped with RFID tags/readers that allow for experimental manipulation of the density and identity of birds feeding at particular stations. By empirically testing density-dependent models of welfare, the project could provide an increased understanding of the interplay among population density, infectious disease, and various social and environmental characteristics, and in doing so, identify drivers of welfare in wild birds.
