Meet our grantees
Wild Animal Initiative funds academic research on high-priority questions in wild animal welfare.
The goal of our grants program is to fund research that deepens scientific knowledge of the welfare of wild animals in order to better understand how to improve the welfare of as many wild animals as possible, regardless of what causes the threats to their well-being.
We showcase our grantees and their projects here and continuously update this page as new projects are added.
Development of novel measures of welfare in juvenile European starlings exposed to nutritional stress
Grantee: Melissa Bateson
Institution: Newcastle University
Project summary
Nutritional stress causes massive mortality in juvenile European starlings and has lifetime welfare consequences for survivors. The aim of this project is to identify the metabolic “fingerprint” of nutritional stress in starling nestlings and to validate molecular biomarkers that can be used to assess the welfare of wild starlings. This project will use untargeted metabolomics to identify multiple novel biomarkers of exposure to nutritional stress. This “fingerprint” will be validated by testing whether it predicts gold-standard behavioral measures of adult affective experience in a cohort of laboratory-raised birds. The aim will be to identify an applicable panel of metabolites that can be measured from feathers and guano.
Grantee: Melissa Bateson
Institution: Newcastle University, United Kingdom
Grant amount: $60,000
Grant type: Challenge grants
Focal species: Starlings (Sturnidae sp.)
Conservation status: Least concern
Disciplines: Physiology, ornithology
Research location: United Kingdom
Project summary
Juveniles of passerine species such as the European starling (Sturnus vulgaris) experience massive mortality, much of which is caused by direct or indirect effects of nutritional stress. Of birds that survive, many will bear the “scars” of early-life stress that have consequences for their welfare. The aim of this project is to identify the metabolic “fingerprint” of nutritional stress in starling nestlings and to validate sensitive and non-invasive molecular biomarkers that can be used to assess the welfare of wild starlings.
This group has been developing the hypothesis that biomarkers of biological age not only predict future morbidity and mortality, but also reflect the quality of an animal’s cumulative lifetime experience. Existing metrics of biological age, particularly telomere length, require invasive blood samples, and measurements are imprecise, meaning that large sample sizes are currently required to obtain significant effects in epidemiological studies. Estimating biological age based on measuring multiple age-related biomarkers (as is typical in the human aging literature) is likely to be more reliable than using telomere length alone.
This project will use untargeted metabolomics to identify multiple novel biomarkers of exposure to nutritional stress in nestling starlings. This “fingerprint” will be validated by testing whether it predicts gold-standard behavioral measures of adult affective experience in a cohort of laboratory-raised birds. Metabolomics measures thousands of small molecules in one biological sample and can be performed on a range of tissues including blood, hair, and urine. The aim will be to identify an applicable panel of metabolites that can be measured cheaply and easily from feathers and the uric acid component of guano.
Why we funded this project
This project should introduce a novel indicator of long-term welfare that is less invasive, requires fewer resources, and is potentially more reliable than similar existing methods. The PI is a world leader in the field of animal behavior and is the main originator of using biological aging to understand long-term animal welfare, especially in non-model species. For that reason, we are especially confident in this work being high-quality and having great academic reach and influence.
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Integrating individual-level juvenile welfare in dynamic habitats across time and space
Grantee: Tom Luhring
Institution: Wichita State University, Texas State University, and Stephen F. Austin University
Project summary
The project will track four populations of juvenile lesser sirens in Eastern Texas within and across years. Sirens’ health is directly affected by their environment through the impacts of resource availability on body condition and growth rates. Furthermore, sirens show strong size-dependent and seasonal shifts in antagonistic behaviors, which lead to acute injuries. This project will use water-borne corticosterone release rates to investigate changes in stress physiology as a function of changes in the environment experienced by the individual (population density, drought severity index, water temperature, pH, conductivity) across time and space to understand coping capacity. This data will also be used to investigate the welfare impact of an established marking technique compared to a novel machine-learning approach.
Grantee: Tom Luhring
Institutions: Wichita State University, Texas State University, and Stephen F. Austin University, United States
Grant amount: $162,604
Grant type: Challenge grants
Focal species: Sirens (Siren intermedia)
Conservation status: Least concern
Disciplines: Herpetology, physiology, climate science
Research location: United States
Project summary
The project will track individual juvenile lesser sirens (Siren intermedia) within and across years for four populations in Eastern Texas. The lack of a terrestrial life-stage and severely limited overland dispersal ability means that hydrologically isolated pools function as closed populations, facilitating recaptures and simplifying demographic estimates. Siren health is directly impacted by the effects of the environment (e.g., drought conditions) through the impacts of resource availability on body condition and growth rates. Furthermore, sirens show strong size-dependent and seasonal shifts in antagonistic behaviors such as biting which lead to acute injuries.
Aquatic amphibians are especially well-suited for the collection of water-borne stress hormones (corticosterone), which offer the least invasive method of evaluating an integrated measure of corticosterone levels that are passively being released through the skin, gills, feces, and urine. This project will use water-borne corticosterone release rates to investigate changes in stress physiology as a function of changes in the environment experienced by the individual (population density, drought severity index, water temperature, pH, conductivity) across time and space to understand coping capacity. These data will also be used to investigate the welfare impact of an established marking technique compared to a novel approach based on pattern recognition by a machine-learning algorithm.
Why we funded this project
Juvenile mortality is especially high in amphibians, and amphibian welfare in general is a neglected subject. This project should provide proof of concept for a cost-effective approach for assessing welfare at both an individual and population level. The waterborne measurements have the potential to integrate corticosterone over a longer period of time, increasing its reliability as a welfare indicator. Finally, this project will test a novel, non-invasive approach to mark-recapture studies, which could facilitate much better individual-level welfare research for amphibians and other (especially aquatic) animals in the future.
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Thermal imaging to investigate physiological state in altricial nestlings
Grantee: Paul Jerem
Institution: Tufts University
Project summary
Animal stress responses evolved to increase survival, in part by stimulating behaviors that reduce exposure to challenging situations. However, young birds who are entirely reliant on their parents (“altricial”) are incapable of acting to change their circumstances, potentially exposing them to the damaging effects of chronic stress. Such species are known to suppress aspects of their stress physiology during development. However, it remains unclear if other parts of the system remain active and could serve as useful indicators for efforts to improve early life welfare. This project seeks to investigate this possibility in juvenile house sparrows (Passer domesticus) using a novel, non-invasive method for inferring internal state — thermal imaging of body surface temperatures.
Grantee: Paul Jerem
Institution: Tufts University, United States
Grant amount: $37,780
Grant type: Challenge grants
Focal species: House sparrow (Passer domesticus)
Conservation status: Least concern
Disciplines: Physiology, ornithology
Research location: United States
Project summary
Animal stress responses evolved to increase survival, in part by stimulating behaviors that reduce exposure to challenging situations. However, young birds who are entirely reliant on their parents (“altricial”) are incapable of acting to change their circumstances, potentially exposing them to the damaging effects of chronic stress. Such species are known to suppress aspects of their stress physiology during development. However, it remains unclear if other parts of the system remain active and could serve as useful indicators for efforts to improve early life welfare. This project seeks to investigate this possibility in juvenile house sparrows (Passer domesticus) using a novel, non-invasive method for inferring internal state — thermal imaging of body surface temperatures.
Why we funded this project
We especially want to understand juvenile welfare, because in most species that is the most experienced life stage, and often the most vulnerable. However, developmental changes can make it difficult to compare welfare between juveniles and adults. This project will calibrate a non-invasive proxy of welfare that can be applied to both adult and altricial juvenile birds, enabling not only better welfare assessments, but more effective lifetime comparisons. A better understanding of the “shape” of lifetime welfare would in turn inform interventions that might disproportionately affect individuals of certain ages.
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It takes guts to grow in the city: the role of the gut microbiome in the welfare of juvenile urban birds
Grantee: Pablo Capilla-Lasheras
Institution: University of Glasgow
Project summary
Approximately 30 million blue tits hatch every year in UK cities, but around 63% die as juveniles. One reason for this may be that juvenile birds in urban habitats tend to have low-quality diets, causing stunted growth and increased early-life mortality. Research suggests that gut microbiome composition may contain indicators of an animal’s welfare, but this has received little attention in a wild animal context. This project will combine telomere attrition, begging behavior, and other health metrics with a study of juvenile birds’ gut microbiomes to investigate how to improve the welfare of juvenile blue tits in urban areas. It will also test the potential for gut microbiome enrichment to improve the life experience of juvenile birds in urban habitats.
Grantee: Pablo Capilla-Lasheras
Institution: University of Glasgow, United Kingdom
Grant amount: $59,052
Grant type: Challenge grants
Focal species: Blue tits (Cyanistes caeruleus)
Conservation status: Least concern
Disciplines: Physiology, population ecology, animal welfare science, ornithology, animal behavior
Research location: United Kingdom
Publications
Reid, R., et al. (2024). The impact of urbanization on health depends on the health metric, life stage and level of urbanization: a global meta-analysis on avian species. Proceedings of the Royal Society B, 291(2027). https://doi.org/10.1098/rspb.2024.0617
Project summary
Amongst the novel stressors that wildlife face in cities, shifts in diet are prevalent and have negative consequences for welfare, particularly in juveniles. Research clearly shows that juvenile birds in urban habitats have low-quality diets, causing stunted growth and increased early-life mortality. For example, in the UK alone, approximately 30 million blue tits hatch every year in cities, but around 63% of them die as juveniles. This project will study juvenile blue tits and investigate how to improve their welfare in urban areas using an integrative approach that will combine metrics of animal welfare with the study of gut microbiomes. Human and captive animal studies suggest that the gut microbiome composition may contain indicators of an animal’s welfare, but this has received little attention in a wild animal context. This study will combine a physiological welfare indicator (telomere attrition), a behavioral welfare indicator (begging behavior), and other health metrics with a study of juvenile birds’ gut microbiomes to link poor-quality urban diets, gut microbiome composition, and welfare. The project will also test a potential near-term dietary intervention (gut microbiome enrichment) to improve the life experience of juvenile birds growing in urban habitats.
Why we funded this project
Welfare is shaped by, and in turn influences, numerous aspects of an animal’s phenotype. Individual welfare indicators offer only limited insights, but combining indicators across multiple domains is thought to be extremely important for triangulating affective state, or “true” welfare. Because the gut microbiome represents a partially distinct domain, understanding how it relates to welfare could therefore strengthen all of our other indicators when they are used in combination. We were especially interested in this project due to the inclusion of an intervention experiment that could be implemented in the near future. Finally, this project will support an early-career researcher with an interest in wild animal welfare.
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Does population density influence the welfare of wild newts?
Grantee: Luiza Figueiredo Passos
Institution: Liverpool John Moores University
Project summary
This project will explore how the welfare of the widespread great crested newt (Triturus cristatus) is influenced by population density. It will test the hypothesis that optimal conditions for population sustainability could be suboptimal at the individual level, and consequently negative for welfare. The researchers will study the welfare of newts in populations with varying densities throughout England. Welfare will be assessed using the Five Domains Model by collecting measures of health, behavior, and nutrition. Environmental parameters will also be collected to assess the relationship between habitat quality and welfare.
Grantee: Luiza Figueiredo Passos
Institution: Liverpool John Moores University, United Kingdom
Grant amount: $158,060
Grant type: Challenge grants
Focal species: Great crested newt (Triturus cristatus)
Conservation status: Least concern
Disciplines: Physiology, animal behavior, herpetology
Research location: United Kingdom
Project summary
The project seeks to determine how the welfare of the widespread species of great crested newts, Triturus cristatus, is influenced by population density in order to help determine how management practices can foster the welfare of individuals. Based on previous studies that have shown that population size and density correlate negatively with body condition and adult survivorship, this study will test the hypothesis that optimal conditions for population sustainability (i.e., conditions that result in higher juvenile survival) could be suboptimal at the individual level, and consequently negative for welfare. The project will study the welfare of newts in populations with varying densities at different locations in England. Welfare will be assessed using the Five Domains model of animal welfare by collecting measures of health (presence of injuries or diseases, corticosterone level, and telomere attrition), behavior (expression of abnormal behavior, flight response, behavioral restriction), and nutrition (body condition, food availability). Environmental parameters (water quality, shelter availability, predator pressure, proximity to anthropogenic threats) will also be collected to assess the relationship between habitat quality and welfare.
Why we funded this project
We funded this project because it will assess density-dependent welfare using a diverse battery of indicators. It is especially exciting to see them applied to a highly abundant amphibian, as the overwhelming majority of wild animal welfare projects have focused on mammals or birds. The proposing team is also very well qualified and has previously been funded by Wild Animal Initiative, which makes them well-suited to take on an analytically challenging project to disentangle influences of population density on newt welfare.
Find Luiza’s other project, studying house sparrows, here.
