Meet our grantees
Wild Animal Initiative funds academic research on high-priority questions in wild animal welfare.
The goal of our grants program is to fund research that deepens scientific knowledge of the welfare of wild animals in order to better understand how to improve the welfare of as many wild animals as possible, regardless of what causes the threats to their well-being.
We showcase our grantees and their projects here and continuously update this page as new projects are added.
Raman spectroscopy as a novel non-invasive technique to assess wildlife welfare
Grantee: Teresa Romero
Institutions: University of Lincoln, University of Portsmouth
Project summary
Analysis of hormones accumulated in the hair has emerged as a non-invasive tool for measuring chronic stress in wildlife, but hair analyses are currently limited by variation in cortisol concentrations. This project will test whether Raman spectroscopy, which is suitable for the field and does not require sample pre-treatment, is a better way to use hair samples to assess steroid hormones. The planned methodology will make use of samples of mammalian hair with a known cortisol content using a control technique — liquid chromatography mass spectrometry — for comparison. The samples will be subjected to analysis by Raman to validate the technique and establish a robust analytical methodology for the non-invasive analysis of welfare biomarkers in wildlife.
Grantee: Teresa Romero
Institution: University of Lincoln, University of Portsmouth, United Kingdom
Grant amount: $19,100
Grant type: Small grants
Focal species: Tufted capuchin monkey (Cebus apella)
Conservation status: Critically endangered
Disciplines: Physiology, animal welfare science
Research location: United Kingdom
Project summary
Steroid hormones are routinely used as biomarkers of stress and can be measured in different biological matrices, such as serum, saliva, feces, and urine. However, commonly used collection methods in wildlife are challenging; the stress they induce in the animals may affect hormone levels and also presents ethical issues. More recently, analysis of hormones accumulated in the hair has emerged as a non-invasive tool for measuring chronic stress in wildlife, but current limitations of hair analyses include variation in cortisol concentrations depending on sample preparation and the amount of hair required for cortisol extraction. This project will test whether Raman spectroscopy, which is suitable for the field and does not require sample pre-treatment, is a better way to use hair samples to assess steroid hormones. The planned methodology will make use of samples of mammalian hair with a known cortisol content using a control technique — liquid chromatography mass spectrometry — for comparison. The samples will be subjected to analysis by Raman to validate the technique and establish a robust analytical methodology for the non-invasive analysis of welfare biomarkers in wildlife.
Why we funded this project
Although this project is focused on development of chemical methods, we expect that the method, if validated, could make glucocorticoid assessment in the hair of wild animals much easier, increasing the future quantity and quality of that type of data. Hair is an especially interesting medium for glucocorticoid analysis because it integrates glucocorticoid levels in the body over time, causing the measurements to be more stable over time and potentially more indicative of baseline welfare, since the values are less sensitive to an animal’s most recent activities.
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Improving the welfare of translocated individuals - European mink as a case study
Grantee: Maria Diez Leon
Institution: University of London
Project summary
This project will assess how levels of two behaviors thought to track positive and negative welfare states — play behaviors and abnormal repetitive behaviors — influence post-release welfare metrics in two on-going reintroduction programs for the European mink. Fecal glucocorticoid metabolite levels will be measured to validate these behavioral indicators pre- and post-release. By differentially raising mink in conditions known to improve welfare, the researchers will investigate whether captive environments that promote play and decrease abnormal repetitive behaviors improve pre-release and post-release welfare, whether individual welfare state pre-release correlates with post-release welfare outcomes, and how the welfare of captive-born individuals differs from that of wild-born individuals.
Grantee: Maria Diez Leon
Institution: University of London, United Kingdom
Grant amount: $28,965
Grant type: Small grants
Focal species: European mink (Mustela lutreola)
Conservation status: Critically endangered
Disciplines: Wildlife rehabilitation, animal welfare science
Research location: Spain
Project summary
Conservation breeding programs do not proactively consider or even assess welfare across all stages, nor track the welfare of released individuals. We therefore lack data on how individual welfare state pre-release might affect welfare post-release. This project aims to fill the gap by assessing how levels of two behaviors thought to track positive and negative welfare states — play behaviors and abnormal repetitive behaviors — influence post-release welfare metrics in two on-going reintroduction programs for the European mink, and compare the welfare of released minks to individuals in an established “benchmark” wild population. Fecal glucocorticoid metabolite levels will also be measured to validate these behavioral indicators pre- and post-release. By differentially raising mink in conditions known to improve welfare, the researchers will investigate whether captive environments that promote play and decrease abnormal repetitive behaviors improve pre-release and post-release welfare, whether individual welfare state pre-release correlates with post-release welfare outcomes, and how the welfare of captive-born individuals differs from that of wild-born individuals.
Why we funded this project
We are interested in the comparison of long-term welfare outcomes between individuals born and protected in captivity and individuals born in the wild, which can help us understand how welfare issues differ between captive and wild environments and how much early-life experiences influence long-term welfare. The project’s objective of identifying improvements for the early-life care of animals who are to be released into the wild also helps us assess “headstarting,” a practice in which juveniles of a species are reared in captivity before being released at a less dangerous life stage, as a near-term intervention for improving the lifetime welfare of animals who have vulnerable juvenile stages but are relatively long-lived as adults. Finally, the applicant has a background in conservation but has demonstrated a long-term interest in wild animal welfare, such as attending an April 2022 workshop on animal sentience (LSE/Rethink Priorities).
Find Maria’s other project, studying American minks and Eurasian otters, here.
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The impact of anthropogenic stressors on the affective state of juvenile Murray cod
Grantee: Rafael Freire
Institution: Charles Sturt University
Project summary
Many freshwater fish populations have severely declined as a result of human-caused changes in their environment. Population decline often results from an increased mortality rate experienced on the individual level, with likely implications for the welfare of individuals living through a period of population decline. This study will examine how differences in water quality and the presence of potential predators affect a behavioral indicator of welfare — judgment bias — in juvenile Murray cod (Maccullochella peelii). In the future, data about how juvenile fish respond to these factors could guide interventions for helping juvenile fish survive to adulthood in the wild.
Grantee: Rafael Freire
Institution: Charles Sturt University, Australia
Grant amount: $21,500
Grant type: Challenge grants
Focal species: Murray cod (Maccullochella peelii)
Conservation status: Critically endangered
Disciplines: Animal behavior, sentience, ichthyology
Research location: Australia
Publications
Freire, R. and Nicole, C.J. (2024). A novel method to measure the impact of water quality on judgement bias in wild juvenile fish. Global Ecology and Conservation, 54. https://doi.org/10.1016/j.gecco.2024.e03086
Project summary
Many freshwater fish populations have severely declined as a result of human-caused changes in their environment. Population decline often results from an increased mortality rate experienced on the individual level, with likely implications for the welfare of individuals living through a period of population decline. This study will examine how differences in water quality and the presence of potential predators affect a behavioral indicator of welfare — judgment bias — in juvenile Murray cod (Maccullochella peelii). In the future, data about how juvenile fish respond to these factors could guide interventions for helping juvenile fish survive to adulthood in the wild.
Why we funded this project
The vast majority of wild fish do not survive to adulthood, but little is known about their welfare as juveniles and how that might affect their survival. This project will address that by investigating the effects of multiple aspects of habitat quality on the affective state of juvenile Murray cod. An additional factor in us funding this project was that it would be integrated into ongoing fisheries policy work by the PI, which should ultimately lead to advice for the regional government.
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