Meet our grantees
Wild Animal Initiative funds academic research on high-priority questions in wild animal welfare.
The goal of our grants program is to fund research that deepens scientific knowledge of the welfare of wild animals in order to better understand how to improve the welfare of as many wild animals as possible, regardless of what causes the threats to their well-being.
We showcase our grantees and their projects here and continuously update this page as new projects are added.
Towards welfare-centered prescribed fire
Grantee: Dale Nimmo
Institution: Charles Sturt University, Australia
Project summary
This project will investigate how prescribed burning affects wild animal welfare, and whether welfare state helps determine survival and harm suffered. The team will study bush rats (Rattus fuscipes) in forest blocks scheduled for prescribed burns and in control sites, combining behavioral, body-condition, injury, and non-invasive physiological measures to infer welfare changes. Lightweight telemetry will enable tracking of survival and severe harm, while fire-severity mapping, refuge measurements, and camera traps will help identify the mechanisms involved. The goal is to generate evidence to help burns be planned and implemented in ways that reduce avoidable suffering.
Grantee: Dale Nimmo
Institution: Charles Sturt University, Australia
Grant amount: $98,700
Grant type: Challenge grants
Focal species: Bush rats (Rattus fuscipes) and others
Conservation status: N/A: Multiple focal species
Disciplines: Climate science, physiology, animal behavior, mammalogy
Research location: Australia
Project summary
This project will investigate how prescribed burning affects the welfare of wild animals, and whether an animal’s welfare state before exposure to fire helps determine survival and harm suffered. The research team will study bush rats (Rattus fuscipes) in forest blocks scheduled for prescribed burns and in control sites, sampling individuals before fire, shortly after fire, and during early recovery, combining behavioral, body-condition, injury, and non-invasive physiological measures to infer changes in affective state and welfare. Lightweight telemetry will enable tracking of survival and severe harm through the burn window and post-fire period, while fire-severity mapping, refuge measurements, and camera traps will help identify the mechanisms involved. The goal is to generate practical evidence for welfare-centered prescribed fire: burns planned and implemented in ways that retain refuges, maintain escape options, and reduce avoidable suffering.
Why we funded this project
By combining welfare indicators with telemetry, fire-exposure metrics, refuge measurements, and predator activity, the project helps move wild animal welfare beyond descriptive assessment toward identifying the mechanisms and management choices that could reduce suffering.
Environmental drivers of welfare in urban rats: A multimodal field study of micro-habitat conditions and affective behavior
Grantee: Emily Mackevicius
Institution: Basis Research Institute, United States
Project summary
This project will investigate how urban micro-habitat conditions shape the affective and social lives of brown rats (Rattus norvegicus). Better tools for understanding rat behavior could support both wild animal welfare science and integrative management practices. The project will first test whether indicators developed in laboratory rodents such as ultrasonic vocalizations, posture, movement, social behavior, and surface temperature can be used to infer affective state and social experience in urban field settings. The team will then model how environmental features predict these indicators, with the goal of identifying urban conditions associated with positive or negative experiences.
Grantee: Emily Mackevicius
Institution: Basis Research Institute, United States
Grant amount: $100,000
Grant type: Challenge grants
Focal species: Brown rat (Rattus norvegicus) and others
Conservation status: Least concern
Disciplines: Wildlife management, physiology, animal behavior
Research location: United States
Project summary
This project will investigate how urban micro-habitat conditions shape the affective and social lives of free-ranging brown rats (Rattus norvegicus). Rats are highly social, intelligent, and abundant animals, and better tools for understanding their behavior could support both wild animal welfare science and integrative management practices. The project will first test whether indicators developed in laboratory rodents such as ultrasonic vocalizations, posture, movement, social behavior, and surface temperature, can be used to infer affective state and social experience in urban field settings. The team will then model how environmental features predict these indicators, with the goal of identifying urban conditions associated with positive or negative experiences.
Why we funded this project
This project addresses a neglected but important question: how fine-scale features of human-built environments shape the subjective experiences of abundant urban animals. The resulting methods could support future welfare studies across sites, cities, and taxa, and provide tools for welfare-aware, evidence-based urban management.
Validating acoustic monitoring as a non-invasive welfare indicator in urban coyotes: Linking vocalizations to health status, social dynamics, and environmental quality
Grantee: Caroline Rowley
Institution: Duke University, United States
Project summary
Urban coyotes face welfare challenges including disease, parasites, habitat degradation, reliance on anthropogenic food sources, and stress from human-wildlife conflict. This project is a pilot study aimed at validating passive acoustic monitoring as a welfare assessment tool. Data will be collected via GPS collars fitted with microphones, autonomous recording units, thermal camera traps and biological sampling. Using models and machine learning, the researchers will test whether vocal features correlate with established welfare indicators and whether individual coyotes can be identified from their howls alone. The aim is to develop a scalable, non-invasive framework for monitoring the welfare of free-ranging animals.
Grantee: Caroline Rowley
Institution: Duke University, United States
Grant amount: $49,978
Grant type: Discovery grants
Focal species: Eastern coyote (Canis latrans)
Conservation status: Least concern
Disciplines: Animal behavior, mammalogy
Research location: United States
Project summary
Coyote populations have expanded dramatically across North America, but population-level success does not necessarily equate to good welfare. Urban coyotes in particular face welfare challenges including disease, parasites, habitat degradation, reliance on anthropogenic food sources, and stress from human-wildlife conflict. This project is a pilot study aimed at validating passive acoustic monitoring as a welfare assessment tool. Data will be collected via GPS collars fitted with microphones, autonomous recording units, thermal camera traps and biological sampling. Using models and machine learning, the researchers will test whether vocal features correlate with established welfare indicators and whether individual coyotes can be identified from their howls alone. The goal is to develop a scalable, non-invasive framework for monitoring the welfare of free-ranging animals.
Why we funded this project
By systematically linking acoustic features of coyote vocalizations to established welfare indicators across multiple domains, this study positions passive acoustic monitoring as a scalable, whole-animal welfare assessment tool that is likely transferable to other similar species using vocalizations for communication.
Comparative assessment of capybara (Hydrochoerus hydrochaeris) welfare in protected, semi-anthropized, and anthropized environments in Argentina
Grantee: Débora Silvia Racciatti
Institution: Universidad de Buenos Aires, Argentina
Project summary
This project will assess how different levels of human disturbance are associated with behavioral, physiological, and health-related welfare indicators in capybara (Hydrochoerus hydrochaeris). Using camera traps, direct observations, non-invasive fecal sampling, and body condition assessments, the team will evaluate a suite of indicators and test not only whether they vary in predicted directions across environments, but how consistently they converge across welfare domains, identifying which measures most reliably discriminate conditions of higher versus lower welfare in free-ranging populations.
Grantee: Débora Silvia Racciatti
Institution: Universidad de Buenos Aires, Argentina
Grant amount: $45,400
Grant type: Discovery grants
Focal species: Capybara (Hydrochoerus hydrochaeris)
Conservation status: Least concern
Disciplines: Human-wildlife conflict, physiology, animal behavior, mammalogy
Research location: Argentina
Project summary
This project compares capybara (Hydrochoerus hydrochaeris) populations living in protected, semi-anthropized, and highly anthropized environments in Argentina to assess how different levels of human disturbance are associated with behavioral, physiological, and health-related welfare indicators. Using camera traps, direct observations, non-invasive fecal sampling, and body condition assessments, the team will evaluate a suite of indicators and test not only whether they vary in predicted directions across environments, but how consistently they converge across welfare domains, identifying which measures most reliably discriminate conditions of higher versus lower welfare in free-ranging populations. By integrating validated measures into a comparative welfare assessment framework, the project aims to advance the empirical foundations of wild animal welfare science and generate evidence to inform welfare-aware wildlife management and coexistence strategies.
Why we funded this project
This project addresses a critical but underexplored question in wild animal welfare science: how anthropogenic landscape transformations affect not just population-level metrics, but also the welfare of individual wild animals. It provides a methodological proof of concept by evaluating a multi-domain suite of non-invasive behavioral, physiological, and health-related indicators that can be validated under real-world field conditions, explicitly assessing their sensitivity, directionality, and cross-domain convergence across contrasting anthropogenic contexts.
Does DNA methylation reflect environmental and social adversity?
Grantee: Daniel T. Blumstein
Institution: University of California, Los Angeles
Project summary
This project will investigate the welfare effects of early cumulative adversity in free-living yellow-bellied marmots. It will look for associations between the adversity index and behaviors that indicate general wariness (flight initiation distance, time allocated to vigilance while foraging, the propensity to emit alarm calls while foraging); biomarkers that indicate physiological stress (fecal glucocorticoid levels, neutrophil:lymphocyte ratios); and two measures of aging (telomere length and DNA methylation). By quantifying these behavioral indices of wariness and the suite of biomarkers that culminate in telomeres and epigenetic state in pups throughout their first year and in older animals throughout their lives, the project will determine whether adverse environmental experiences have immediate and lasting effects on welfare.
Grantees: Daniel T. Blumstein, Emily Renkey
Institution: University of California, Los Angeles, US
Grant amount: $219,900
Grant type: Challenge grants
Focal species: Yellow-bellied marmot (Marmota flaviventer)
Conservation status: Least concern
Disciplines: Population ecology, genetics/genomics, ecological modeling, physiology
Research locations: United States
Project summary
In humans, early cumulative adversity has demonstrable consequences for health, welfare, and longevity. The welfare consequences of early adversity in wild animals is less well understood, possibly reflecting insufficient measures for assessment. Using a recently validated approach to quantifying cumulative adversity in free-living yellow-bellied marmots (Marmota flaviventer), this project will investigate the welfare effects of early cumulative adversity. It will look for associations between the adversity index and key behaviors that indicate general wariness (flight initiation distance, time allocated to vigilance while foraging, and the propensity to emit alarm calls while foraging), biomarkers that indicate physiological stress (fecal glucocorticoid levels, neutrophil:lymphocyte ratios), and ultimately, two measures of aging (telomere length and DNA methylation). By quantifying these behavioral indices of wariness and the suite of biomarkers that culminate in telomeres and epigenetic state in pups throughout their first year and in older animals throughout their lives, the project will determine whether adverse environmental experiences have immediate and lasting effects on welfare.
Why we funded this project
This project will add welfare to the research portfolio of a long-running study system of a free-living mammal. It will contribute to understanding the validity of biological aging as a welfare indicator by pairing it with other indicators and a comprehensive dataset of the animals’ adverse early-life experiences.
Remote welfare assessment in wildlife using stand-off Raman spectroscopy
Grantees: Jose Gonzalez-Rodriguez and Teresa Romero
Institution: University of Lincoln
Project summary
This project aims to develop a stand-off Raman spectroscopy system as a non-invasive tool to assess hormonal levels in wild animals, which can be integrated into welfare assessments. The project also aims to validate under standardized and natural conditions how biomarkers of long-term stress relate to a range of stressors that may affect wild animal welfare. They will use a combination of socio-positive (e.g., play, grooming) and negative (e.g., aggression, screaming) behaviors, indices of social integration, behavioral indicators of anxiety (i.e., self-directed behaviors), and resting behavior, as well as physical indicators of welfare (body condition, instances of injury).
Grantees: Jose Gonzalez-Rodriguez and Teresa Romero
Institution: University of Lincoln, UK
Grant amount: $55,519
Grant type: Discovery grants
Focal species: Tufted capuchin monkeys (Sapajus apella)
Conservation status: Least concern
Disciplines: Physiology, animal behavior
Research locations: United Kingdom, United States
Project summary
To enhance the welfare of wild animals, it is crucial to continuously monitor biomarkers and other metrics that can indicate changes in their welfare. However, this presents the challenge of obtaining repeated measurements from individuals, which often involves capture, restraint, and/or handling — procedures that can have significant negative welfare impacts on free-living wild animals. This project aims to develop a stand-off Raman spectroscopy system as a non-invasive tool to assess hormonal levels in wild animals, which can be integrated into welfare assessments. The project also aims to validate under standardized and natural conditions how biomarkers of long-term stress relate to a range of stressors that may affect wild animal welfare. They will use a combination of socio-positive (e.g., play, grooming) and negative (e.g., aggression, screaming) behaviors, indices of social integration, behavioral indicators of anxiety (i.e., self-directed behaviors), and resting behavior, as well as physical indicators of welfare (body condition, instances of injury).
Why we funded this project
This project builds on a previous WAI-funded project, which validated the use of Raman spectroscopy as an efficient way of testing hormones in hair. It will test whether this method can be used to measure hair cortisol levels from a distance, potentially helping to make the use of this indicator more scalable for long-term monitoring in the field, and reducing the need for distressing or invasive methods for welfare assessment.
Validating body posture as a novel marker of well-being in animals
Grantee: Nicola Koyama
Institution: Liverpool John Moores University
Project summary
A few studies on nonhuman primates have found that a hunched posture is a response to social separation and physical inflammation, but research linking whole body posture and physiological measures of welfare is lacking. This project will combine established measures of affective valence (nasal temperature) and arousal (iris-pupil ratio) to validate body posture as a new measure of affective valence. Two months of video data will be collected and used for thermal imaging, behavioral, and postural analysis from groups of wild Barbary macaques, along with pilot data for comparison from non-human primate zoo animals. Ultimately, the project aims to share a new validated measure and conceptual framework that can be applied to a range of wild mammals.
Grantee: Nicola Koyama
Institution: Liverpool John Moores University, United Kingdom
Grant amount: $9,995
Grant type: Seed grants
Focal species: Barbary macaque (Macaca sylvanus)
Conservation status: Endangered
Disciplines: Primatology, animal behavior, physiology
Research locations: United Kingdom, Morocco
Project summary
A few studies on non-human primates have found that a hunched posture is a response to social separation and physical inflammation, but research linking whole body posture and physiological measures of welfare is lacking. This project will combine established measures of affective valence (nasal temperature) and arousal (iris-pupil ratio) to validate body posture as a new measure of affective valence. Two months of video data will be collected and used for thermal imaging, behavioral, and postural analysis from groups of wild Barbary macaques, along with pilot data for comparison from non-human primate zoo animals. Ultimately, the project aims to share a new validated measure and conceptual framework that can be applied to a range of wild mammals.
Why we funded this project
This project will validate an indicator for assessing welfare that could be relevant across primates, and provide proof of concept for its application in the field.
An Evaluation of Effective Disturbance Mitigation Measures for European Badgers (Meles meles) Occupying Setts During Forestry Operations
Grantee: Stephen McAuliffe
Institution: University of Brighton and Forest Research
Project summary
In order to avoid possible disturbance to European badgers, forestry guidance in England states that activities using heavy machinery should be prohibited within 20 meters of active badger setts. This research aims to validate that the approved noise and vibration mitigation buffer distances intended to prevent disturbance from anthropogenic activities are effective. Observations of badger behavior will be made in woodland habitats, and fecal cortisol metabolites will be collected for analysis. If changes in behavior and increases in cortisol levels are not found following exposure to forestry noise and vibration greater than 20 meters away, robust evidence will be provided to validate that badger welfare is not being adversely impacted by legally sanctioned forestry activities.
Grantee: Stephen McAuliffe
Institution: University of Brighton and Forest Research, United Kingdom
Grant amount: $10,137
Grant type: Seed grants
Focal species: European badger (Meles meles)
Conservation status: Least concern
Disciplines: Animal behavior, physiology, mammalogy, human-wildlife conflicet
Research locations: United Kingdom
Project summary
In order to avoid possible disturbance to European badgers (Meles meles), forestry guidance in England states that activities using heavy machinery should be prohibited within 20 meters of active badger setts. However, this commonly adopted mitigation measure has never been scientifically assessed. As a result, it relies on the untested assumption that noise and vibrations do not disturb badgers if a buffer zone of 20 meters is maintained between forestry operations and setts. This research aims to validate that the approved noise and vibration mitigation buffer distances intended to prevent disturbance from anthropogenic activities are effective. Observations of badger behavior will be made in woodland habitats, and fecal cortisol metabolites will be collected for analysis. If changes in behavior and increases in cortisol levels are not found following exposure to forestry noise and vibration greater than 20 meters away, robust evidence will be provided to validate that badger welfare is not being adversely impacted by legally sanctioned forestry activities.
Why we funded this project
This project will help us understand the welfare impacts of anthropogenic disturbance on a common mammal, and could lead to a very near-term intervention to mitigate them if the project results in a recommendation to increase buffer distances. We hope it will set a precedent for testing and improving animal welfare protection methods routinely advised and adopted by many land-based sectors such as forestry, utilities providers, construction and agriculture.
Parasites in the city: How is the impact of parasites on wild animal welfare affected by urbanization?
Grantee: Amanda Trask
Institutions: Institute of Zoology (IOZ), Zoological Society of London (ZSL)
Project summary
This project will evaluate the extent to which parasitism and urbanization impact wild animal welfare, both independently and synergistically, using physiological data on the internal and external parasite loads of deceased gray squirrels, as well as their body condition, immune function, and stress response. The researchers will also collect behavioral welfare indicators from other squirrels using camera traps. This data will be collected along a gradient of urbanization levels and analyzed in the context of the squirrels’ sex and age, to compare population-level welfare across sites varying in degree of urbanization and average parasite metrics and predict how future increases in urbanization might impact wildlife welfare.
Grantee: Amanda Trask
Institutions: Institute of Zoology (IOZ), Zoological Society of London (ZSL), United Kingdom
Grant amount: $199,941
Grant type: Challenge grant
Focal species: Eastern grey squirrel (Sciurus carolinensis)
Conservation status: Least concern
Disciplines: Infectious disease, population ecology
Research location: United Kingdom
Project summary
Parasitism is likely a key determiner of wild animal welfare, while rapidly increasing urbanisation presents novel challenges and welfare threats, yet the extent that parasitism and urbanisation impact wild animal welfare, both independently and synergistically, remains a major knowledge gap. An individual’s parasite infection status, and the associated welfare impacts, is dependent on parasite exposure risk and susceptibility. Urbanisation may alter exposure risk, for example through changes to population density and resource distribution, and susceptibility, for example through altered host immune function from exposure to urban stressors. Urbanisation may also compound impacts of parasitism, if chronically stressed individuals are less able to cope with further welfare challenges, resulting in synergistic negative welfare effects. Furthermore, welfare impacts of parasites and urbanisation may vary across sex- and life-stage classes, for example due to hormonal influences and changes in immune function with chronic exposure to urban pollution.
We will address this knowledge gap, using grey squirrels from UK sites across an urbanisation gradient. We will use detailed individual-level data on parasite loads and physical and physiological welfare indicators, collected from dead squirrels killed as part of non-native species management, to determine welfare impacts across sites varying in degree of urbanisation, and across sexes and life stages. Then, we will use behavioural indicators from camera trap data to determine associations between population-level welfare, parasite prevalence and degree of urbanisation. Finally, we will estimate ‘welfare expectancy’ of individuals from different populations using sex- and life-stage specific welfare scores. We will expand on current theory and develop a modelling tool to incorporate demographic and environmental stochasticity in welfare states into population models, to aid prediction of how increasing urbanisation may impact wildlife welfare in the future. This study will provide vital knowledge to aid understanding and prediction of the experience and future responses of wild animals to parasites in the context of increasing urbanization, facilitating design and integration of welfare-friendly urban environments for wildlife.
Why we funded this project
Gray squirrels are abundant, they live in close proximity to humans, and they could benefit from welfare interventions such as wildlife fertility control in the near future. Gray squirrels are not native in the UK, where this study will be conducted, and the welfare of non-native species is generally understudied or undervalued compared with native species of conservation interest. This project provides a special opportunity to take advantage of data from squirrels who are being killed as part of a government program, and to access valuable metrics that are hard to measure non-invasively, thereby creating from their deaths an opportunity to gain valuable insights that could ultimately help other squirrels. In addition to their good range of welfare indicators, we appreciate that this project will consider sex- and age-specific variation in welfare impacts and attempt to estimate “welfare expectancy” at a population level.
Are we making urban wildlife sick?
Grantee: Carl Soulsbury
Institution: University of Lincoln
Project summary
The goal of this project is to test whether differences in welfare among individual red foxes and individual European hedgehogs are explained by specific environmental stressors arising from urbanization (e.g., increased human population density, noise pollution, or reduced habitat heterogeneity). Notably, on a population level, foxes appear to have fared well alongside humans, while hedgehogs have struggled to cope with urbanization. The study will confirm whether this contrast is also seen at the level of individual welfare, and offers a unique opportunity to identify common environmental stressors that may be impacting the health and welfare of urban wildlife more generally. To assess welfare, the project will perform cognitive judgment bias and novel object tests and combine them with non-invasive indicators of physiological stress (fecal glucocorticoids and eye/ear temperature) and health (pathogen load).
Grantee: Carl Soulsbury
Institutions: University of Lincoln, United Kingdom
Grant amount: $148,741.43
Grant type: Challenge grant
Focal species: Red fox (Vulpes vulpes) and European hedgehog (Erinaceus europaeus)
Conservation status: Least concern
Disciplines: Animal welfare science, animal behavior, physiology
Research location: United Kingdom
Project summary
Urbanisation causes profound changes to biodiversity. For many species living in urban areas, there is evidence that urban living leads to greater disease prevalence. It is unclear, however, (a) what factors drive this higher disease prevalence and (b) whether this leads to poorer wild animal welfare. We propose a study to compare two urban-dwelling species, red foxes and Eurasian hedgehogs, where we will characterize pathogen prevalence, load, and diversity across an urban-rural gradient. This study will be carried out at two scales: firstly, we will link pathogen loads, faecal glucocortoicoids and habitat at the landscape level, allowing us to broadly understand if urbanisation is associated with increased pathogen loads. We will then carry out a second, more detailed data collection where we will combine pathogen data with measures of stress (infra-red thermography, faecal glucocorticoids) and behavioural measures of affective valence (novel object and judgement bias tests). Here, we will link together pathogen loads and different measures of welfare at the individual level. Ultimately, our aim is to test, within each species, whether population differences in health and welfare are explained by environmental stressors arising from urbanisation (e.g., increased human population density and reduced habitat heterogeneity).
Why we funded this project
We appreciate that this project considers specific characteristics of urban environments, as opposed to simply comparing urban environments with rural ones. This way, it has more potential to inform near-term interventions. Plus, the project design humanely allows for animals’ voluntary participation. In addition to using an effective suite of welfare indicators to investigate the impact of different parasite loads on welfare, the combination of cognitive bias testing with non-invasive physiological stress metrics will provide information on the validity of those metrics, which are being used concurrently in other projects.
Find Carl’s other project, studying wild insects, here.