Meet our grantees
Wild Animal Initiative funds academic research on high-priority questions in wild animal welfare.
The goal of our grants program is to fund research that deepens scientific knowledge of the welfare of wild animals in order to better understand how to improve the welfare of as many wild animals as possible, regardless of what causes the threats to their well-being.
We showcase our grantees and their projects here and continuously update this page as new projects are added.
Determining the practical and statistical methods necessary for employing field-based metrics of welfare on wild, juvenile, birds
Grantee: Daniel Hanley
Institution: George Mason University
Project summary
Because welfare can vary between individuals and throughout the life of an animal, methods for measuring, assessing, and comparing welfare have been a barrier to our understanding of juvenile welfare. Initial investigations of welfare metrics are needed to estimate age-specific welfare in wild juvenile animals, to determine how they deviate from population-level estimates, and to extend methods and metrics to other systems. This study will examine welfare in free-living prothonotary warblers to establish standardized field and analytical procedures necessary to obtain age-specific animal welfare estimates. Prothonotary warblers are an ideal model system for studying age-specific welfare because they have well-defined life stages, face unique environmental risks, have variable survival, and nest within cavities, affording a degree of standardization and control.
Grantee: Daniel Hanley
Institution: George Mason University, United States
Grant amount: $60,000
Grant type: Challenge grants
Focal species: Prothonotary warbler (Protonotaria citrea)
Conservation status: Least concern
Disciplines: Ornithology, animal behavior, population ecology
Research location: United States
Project summary
The juvenile stage is where welfare conditions are likely the most variable and impactful on an individual’s growth and behavior. Unfortunately, methods for measuring, assessing, and comparing welfare have been a barrier to our understanding of juvenile welfare. Like other aspects of animal life history, welfare will vary between individuals and also over the lives of animals in an age-specific fashion. Thus, metrics such as welfare expectancy can inform us of the welfare that an organism is likely to experience, similar to how life expectancy can provide an estimate on how much longer an organism may live.
This study examines welfare in free-living juvenile songbirds to establish standardized field and analytical procedures necessary to obtain age-specific animal welfare estimates. Prothonotary warblers are an ideal model system for studying age-specific welfare because they have well-defined life stages (i.e., egg, nestling, fledgling, subadult, adult), face unique environmental risks (e.g., drought and flooding), and have variable survival. Furthermore, members of this species nest within cavities, which affords a degree of standardization and control necessary for an initial investigation of welfare metrics. Such initial investigations are crucial to estimate age-specific welfare on wild juvenile animals, to determine how they deviate from population-level welfare estimates, and to extend these methods and metrics to other systems.
Why we funded this project
We funded this project because it sought to explicitly quantify welfare across life stages, using multiple physiological, behavioral, and environmental/demographic indicators. Knowing how (and ideally why) average welfare differs over the course of life in a population could have important implications for interventions to improve their welfare (e.g., fertility control). We were also impressed with this PI because he engages numerous students in their lab and is relatively early in his own career, potentially allowing for pivot to focus more on wild animal welfare. He also demonstrated a good understanding of Wild Animal Initiative’s research on the welfare expectancy framework and sought to put the concepts into practice. That sort of theory-to-practice pipeline would represent a significant step for welfare biology as a research field.
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It takes guts to grow in the city: the role of the gut microbiome in the welfare of juvenile urban birds
Grantee: Pablo Capilla-Lasheras
Institution: University of Glasgow
Project summary
Approximately 30 million blue tits hatch every year in UK cities, but around 63% die as juveniles. One reason for this may be that juvenile birds in urban habitats tend to have low-quality diets, causing stunted growth and increased early-life mortality. Research suggests that gut microbiome composition may contain indicators of an animal’s welfare, but this has received little attention in a wild animal context. This project will combine telomere attrition, begging behavior, and other health metrics with a study of juvenile birds’ gut microbiomes to investigate how to improve the welfare of juvenile blue tits in urban areas. It will also test the potential for gut microbiome enrichment to improve the life experience of juvenile birds in urban habitats.
Grantee: Pablo Capilla-Lasheras
Institution: University of Glasgow, United Kingdom
Grant amount: $59,052
Grant type: Challenge grants
Focal species: Blue tits (Cyanistes caeruleus)
Conservation status: Least concern
Disciplines: Physiology, population ecology, animal welfare science, ornithology, animal behavior
Research location: United Kingdom
Publications
Reid, R., et al. (2024). The impact of urbanization on health depends on the health metric, life stage and level of urbanization: a global meta-analysis on avian species. Proceedings of the Royal Society B, 291(2027). https://doi.org/10.1098/rspb.2024.0617
Project summary
Amongst the novel stressors that wildlife face in cities, shifts in diet are prevalent and have negative consequences for welfare, particularly in juveniles. Research clearly shows that juvenile birds in urban habitats have low-quality diets, causing stunted growth and increased early-life mortality. For example, in the UK alone, approximately 30 million blue tits hatch every year in cities, but around 63% of them die as juveniles. This project will study juvenile blue tits and investigate how to improve their welfare in urban areas using an integrative approach that will combine metrics of animal welfare with the study of gut microbiomes. Human and captive animal studies suggest that the gut microbiome composition may contain indicators of an animal’s welfare, but this has received little attention in a wild animal context. This study will combine a physiological welfare indicator (telomere attrition), a behavioral welfare indicator (begging behavior), and other health metrics with a study of juvenile birds’ gut microbiomes to link poor-quality urban diets, gut microbiome composition, and welfare. The project will also test a potential near-term dietary intervention (gut microbiome enrichment) to improve the life experience of juvenile birds growing in urban habitats.
Why we funded this project
Welfare is shaped by, and in turn influences, numerous aspects of an animal’s phenotype. Individual welfare indicators offer only limited insights, but combining indicators across multiple domains is thought to be extremely important for triangulating affective state, or “true” welfare. Because the gut microbiome represents a partially distinct domain, understanding how it relates to welfare could therefore strengthen all of our other indicators when they are used in combination. We were especially interested in this project due to the inclusion of an intervention experiment that could be implemented in the near future. Finally, this project will support an early-career researcher with an interest in wild animal welfare.
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The impact of road noise on the welfare of free-living juvenile white-footed mice
Grantee: Michael Sheriff
Institution: University of Massachusetts Dartmouth
Project summary
This project will examine how road noise impacts the ability of juvenile white-footed mice in Massachusetts to respond to the threat of predation. Preliminary work has shown that experimental manipulation of road noise disrupts the foraging responses of (adult) small mammals to predation risk, possibly by masking their ability to perceive predators’ auditory cues. Perception of predation threat will be experimentally manipulated by auditory playback of owl noises at sites near and far from the highway, paired with controls at the same distances from the highway without auditory playback. Anxiety-related behaviors will be recorded in juveniles in an open field trap, and their feces will be studied to assess physiological stress and nutritional status.
Grantee: Michael Sheriff
Institution: University of Massachusetts Dartmouth, United States
Grant amount: $60,000
Grant type: Challenge grants
Focal species: White-footed mice (Peromyscus leucopus)
Conservation status: Least concern
Disciplines: Human-wildlife conflict, animal behavior, population ecology, mammalogy
Research location: United States
Project summary
This project will examine the impact of road noise on juvenile welfare in white-footed mice in Massachusetts. The project will focus on how road noise impacts the ability of juveniles to respond appropriately to the threat of predation (the most common cause of juvenile small mammal mortality). Preliminary work has shown that experimental manipulation of road noise (played at 62-65dB, which is equivalent to 100m into the forest from a major thoroughfare to Boston, MA) disrupts the normal foraging responses of (adult) small mammals to predation risk, possibly by masking their ability to perceive auditory cues of predators. Perception of predation threat will be experimentally manipulated by auditory playback of owl noises at sites near and far from the highway, paired with controls at the same distances from the highway but without auditory playback. Anxiety-related behaviors will be recorded in juveniles in an open field trap (which they voluntarily enter for feed), and their feces will be studied to assess physiological stress and nutritional status.
Why we funded this project
Road noise has dramatically increased and is potentially a major anthropogenic threat to wild animal welfare, and one which might be easily ameliorated through policy changes (e.g., improved sound barriers). This project is especially interesting because it focuses on a less obvious effect of road noise, potentially increasing the risk of predation by masking predator cues. This becomes even more interesting in the context of growing literature on the “ecology of fear,” sublethal effects of predators on prey behavior. If road noise makes prey unaware of risks, it could actually reduce their chronic stress despite exposing them to greater risk of death. We are excited for this project to explore those issues, although we are prepared for a complex result. Additionally, we wanted to support this PI because of their strong record of engaging students in their research and influencing their career trajectories.
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The impact of anthropogenic stressors on the affective state of juvenile Murray cod
Grantee: Rafael Freire
Institution: Charles Sturt University
Project summary
Many freshwater fish populations have severely declined as a result of human-caused changes in their environment. Population decline often results from an increased mortality rate experienced on the individual level, with likely implications for the welfare of individuals living through a period of population decline. This study will examine how differences in water quality and the presence of potential predators affect a behavioral indicator of welfare — judgment bias — in juvenile Murray cod (Maccullochella peelii). In the future, data about how juvenile fish respond to these factors could guide interventions for helping juvenile fish survive to adulthood in the wild.
Grantee: Rafael Freire
Institution: Charles Sturt University, Australia
Grant amount: $21,500
Grant type: Challenge grants
Focal species: Murray cod (Maccullochella peelii)
Conservation status: Critically endangered
Disciplines: Animal behavior, sentience, ichthyology
Research location: Australia
Publications
Freire, R. and Nicole, C.J. (2024). A novel method to measure the impact of water quality on judgement bias in wild juvenile fish. Global Ecology and Conservation, 54. https://doi.org/10.1016/j.gecco.2024.e03086
Project summary
Many freshwater fish populations have severely declined as a result of human-caused changes in their environment. Population decline often results from an increased mortality rate experienced on the individual level, with likely implications for the welfare of individuals living through a period of population decline. This study will examine how differences in water quality and the presence of potential predators affect a behavioral indicator of welfare — judgment bias — in juvenile Murray cod (Maccullochella peelii). In the future, data about how juvenile fish respond to these factors could guide interventions for helping juvenile fish survive to adulthood in the wild.
Why we funded this project
The vast majority of wild fish do not survive to adulthood, but little is known about their welfare as juveniles and how that might affect their survival. This project will address that by investigating the effects of multiple aspects of habitat quality on the affective state of juvenile Murray cod. An additional factor in us funding this project was that it would be integrated into ongoing fisheries policy work by the PI, which should ultimately lead to advice for the regional government.
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Density-Dependent Welfare in Wild Bird Social Networks: Linking resource distributions with disease dynamics
Grantee: Joshua Firth
Institution: University of Oxford
Project summary
This project will investigate how various potential density-dependent drivers of welfare interact and influence net welfare in two species of tit. Population density is expected to be directly related to infectious disease transfer and increased competition, but also to covary with processes that are potentially beneficial to welfare. The study will use historical data to determine how the relationship between population density and individual welfare is shaped by infectious disease, body condition, and mortality risk. The investigators will then manipulate density experimentally to test model outputs and determine potential causal links.
Grantee: Josh Firth
Institution: University of Oxford, United Kingdom
Grant amount: $99,466
Grant type: Challenge grants
Focal species: Great tits (Parus major), blue tits (Cyanistes caeruleus)
Conservation status: Least concern
Disciplines: Animal behavior, community ecology, infectious disease, population ecology, ornithology, bio/eco-informatics, ecological modeling, animal welfare science
Research location: United Kingdom
Project summary
This project seeks to understand the interplay among various potential density-dependent drivers of positive and negative welfare impacts to determine net welfare related to aggregation (increased density) in wild birds (two species of tit). Infectious disease transfer and increased competition, both likely to reduce welfare, are expected to be directly related to population density. However, population density also covaries with processes that are potentially beneficial to welfare, such as congregation around areas that provide high nutrition, various social benefits, opportunities for increased cooperation, or access to social information. The study will use long-term datasets to empirically determine how the relationship between population density and individual welfare is shaped by infectious disease, including the density-dependent relationship with disease spread. Additionally, using historical data, the project will test how population density, mediated by social contact, environment, and disease, determines individuals’ body condition and mortality risk. Based on the results of this observational phase of the study, the investigators will then manipulate density experimentally, to test model outputs and determine potential causal links.
Why we funded this project
This project brings two unique advantages. First, the investigators have access to a population that has been subject to intensive monitoring for decades, meaning that much of the relevant ecological context is known and there is historical data to analyze retrospectively. Secondly, in part due to this long-term monitoring, the birds are tagged and observed at feeding stations equipped with RFID tags/readers that allow for experimental manipulation of the density and identity of birds feeding at particular stations. By empirically testing density-dependent models of welfare, the project could provide an increased understanding of the interplay among population density, infectious disease, and various social and environmental characteristics, and in doing so, identify drivers of welfare in wild birds.
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Drivers of welfare in American pikas (Ochotona princeps)
Grantee: Johanna Varner
Institution: Colorado Mesa University
Project summary
Pikas (Ochotona princeps) in alpine habitats have recently faced population losses and range retractions. Declines are most prominent in isolated mountain ranges with limited high-elevation habitat, like the La Sals, where pikas are consequently likely to experience lower welfare. This project will assess the welfare of pikas in the La Sals through patterns of survival, population demographics, physiological stress, body condition, ectoparasite load, and hydration. The researchers will also test hypotheses correlating these indicators to habitat quality, and investigate resource competition between pikas and mountain goats.
Grantee: Johanna Varner
Institution: Colorado Mesa University, United States
Grant amount: $28,500
Grant type: Small grants
Focal Species: American pikas (Ochotona princeps)
Conservation status: Least concern
Disciplines: Population ecology, animal behavior, ecological modeling, community ecology
Research location: United States
Project summary
Due to recent population losses and range retractions, pikas (Ochotona princeps) are considered a sentinel for climate change in the alpine. However, declines are most prominent in isolated mountain ranges with limited high-elevation habitat, like the La Sals. Surrounded by low-elevation desert in all directions, pikas in the La Sals are likely to experience lower aggregate welfare (i.e., steeper population declines, greater physiological stress, and lower survivorship). This project will assess the welfare of pikas in the La Sals through patterns of survival, population demographics, physiological stress (fecal glucocorticoid metabolites; FGM), body condition, ectoparasite load, and hydration. The researchers will also test hypotheses correlating these welfare indicators to aspects of habitat quality. Finally, they will also investigate resource competition between pikas and mountain goats as another potential factor in pika welfare.
Why we funded this project
This project addresses our research priority of understanding the welfare implications of competition and other conflicts of interest between wild animals. It also focuses on a highly numerous species, the American pika. In that context, we appreciate that this project will model habitat quality on the spatial scale relevant to individual pika when assessing its association with their welfare. Finally, we value that they will use a wide variety of welfare indicators, representing the domains of physiology, behavior, and environment/demography, with each strengthening interpretation of the other.
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Does population density influence the welfare of wild newts?
Grantee: Luiza Figueiredo Passos
Institution: Liverpool John Moores University
Project summary
This project will explore how the welfare of the widespread great crested newt (Triturus cristatus) is influenced by population density. It will test the hypothesis that optimal conditions for population sustainability could be suboptimal at the individual level, and consequently negative for welfare. The researchers will study the welfare of newts in populations with varying densities throughout England. Welfare will be assessed using the Five Domains Model by collecting measures of health, behavior, and nutrition. Environmental parameters will also be collected to assess the relationship between habitat quality and welfare.
Grantee: Luiza Figueiredo Passos
Institution: Liverpool John Moores University, United Kingdom
Grant amount: $158,060
Grant type: Challenge grants
Focal species: Great crested newt (Triturus cristatus)
Conservation status: Least concern
Disciplines: Physiology, animal behavior, herpetology
Research location: United Kingdom
Project summary
The project seeks to determine how the welfare of the widespread species of great crested newts, Triturus cristatus, is influenced by population density in order to help determine how management practices can foster the welfare of individuals. Based on previous studies that have shown that population size and density correlate negatively with body condition and adult survivorship, this study will test the hypothesis that optimal conditions for population sustainability (i.e., conditions that result in higher juvenile survival) could be suboptimal at the individual level, and consequently negative for welfare. The project will study the welfare of newts in populations with varying densities at different locations in England. Welfare will be assessed using the Five Domains model of animal welfare by collecting measures of health (presence of injuries or diseases, corticosterone level, and telomere attrition), behavior (expression of abnormal behavior, flight response, behavioral restriction), and nutrition (body condition, food availability). Environmental parameters (water quality, shelter availability, predator pressure, proximity to anthropogenic threats) will also be collected to assess the relationship between habitat quality and welfare.
Why we funded this project
We funded this project because it will assess density-dependent welfare using a diverse battery of indicators. It is especially exciting to see them applied to a highly abundant amphibian, as the overwhelming majority of wild animal welfare projects have focused on mammals or birds. The proposing team is also very well qualified and has previously been funded by Wild Animal Initiative, which makes them well-suited to take on an analytically challenging project to disentangle influences of population density on newt welfare.
Find Luiza’s other project, studying house sparrows, here.
